Advanced Search

Submit Online

Articles

< Previous                        
Isolation of purified and live Foxp3+ regulatory T cells using FACS sorting on scatter plot Free
Xiaohui Zhou1,2, Julie Wang1, Wei Shi3, David D. Brand4, Zhongmin Liu2, Huimin Fan2,*, and Song Guo Zheng1,*
1Division of Rheumatology and Immunology, Department of Medicine, University of Southern California, Los Angeles, CA, USA
2Center of Immunoregulation and Tolerance, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
3Developmental Biology Program, Department of Surgery, Children's Hospital Los Angeles, University of Southern California, Los Angeles, CA, USA
4Research Service, Veterans Affairs Medical Center, Memphis, TN, USA *Correspondence to:Song Guo Zheng, 2011 Zonal Avenue, Hoffman Medical Research Building, Room 710A, Los Angeles, CA 90033, USA, Tel: +1-323-4422128; Fax: +1-323-4422874; E-mail: szheng@usc.edu; Huimin Fan, Tel: +86-21-58409274; Fax: +86-21-58761951; E-mail: frankfan64@hotmail.com
J Mol Cell Biol, Volume 2, Issue 3, June 2010, 164-169,  https://doi.org/10.1093/jmcb/mjq007
Keyword: Foxp3, TGF-β , regulatory T cells
There are no ideal ways to identify and isolate viable and purified Foxp3+ regulatory T cells so far. Here we developed a novel procedure for the isolation of highly purified Foxp3+ cells using flow cytometry. This method relies on an identification and sorting of the lymphoblast cell population identified on a scatter plot using flow cytometry. We confirmed that greater than 98% of the cells sorted using this technique expressed Foxp3 and displayed a potent suppressive activity. This method provides a valuable tool for the study of the T regulatory cell biology and their therapeutic manipulation.

Volume 2, Issue 3
June 2010